FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924882
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG355690.9062438055462559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT39450.039.6905021
TGGTATC40200.038.5826452
GTATCAA139100.025.5795381
GGTATCA75700.024.6464941
AGAGTAC104900.023.41013111
TATCAAC155400.022.7754252
CAGAGTA111200.022.30697410
ATCAACG165650.021.2812333
TCAACGC166700.021.1453024
CAACGCA168500.020.8522835
AACGCAG172150.020.4253566
AGTACGG118600.020.3583813
GAGTACG121350.020.03299512
CGAATTA6200.019.07261315
TACGGGG54150.018.73964315
CGCAGAG189400.018.5690968
ACGCAGA189350.018.5421187
GTACGGG131250.017.9559914
CCGAATT7100.017.81670414
GCAGAGT200150.017.6420239
Produced by FastQC (version 0.11.5)