FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134747
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT61800.1971450965580316No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.12683639221921258No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.11886126695392005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26950.039.4301071
GTATCAA82650.038.108521
TGGTATC28550.037.069712
GGTATCA44600.036.501491
TATCAAC93200.033.7931142
ATCAACG94250.033.3264473
TCAACGC95550.032.8730244
CAACGCA97650.032.1225555
AACGCAG100250.031.289456
ACGCAGA108450.028.9591247
CGCAGAG111300.028.2553148
GCAGAGT124450.025.337619
CAGAGTA76350.023.26667410-11
ATACCGC2254.129106E-1022.6685986
GAGTACG85400.020.70169612-13
AGAGTAC72250.020.55778910-11
GTACGGG93500.018.658314-15
AGTACGG80950.018.13851412-13
GTATTAG12750.018.0023961
GTCGAGG11350.017.5980918
Produced by FastQC (version 0.11.5)