FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134747
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265330.846415994656028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT30200.035.619921
TGGTATC31450.033.488852
GTATCAA96750.026.4172671
AGAGTAC74350.024.37515311
TATCAAC106000.023.84142
CAGAGTA79850.022.90285510
ATCAACG110950.022.7267023
GGTATCA50500.022.717881
TCAACGC113100.022.2192544
CAACGCA114000.022.089965
AACGCAG114350.021.9735626
AGTACGG81400.021.85862213
GAGTACG85250.021.09727112
ACGCAGA125000.020.1457237
TACGGGT6850.019.67168215
CGCAGAG128350.019.623048
TGCCGAC9550.019.58317655
TACGGGG43700.019.00471915
GTACGGG95550.018.59220314
GCAGAGT136750.018.441029
Produced by FastQC (version 0.11.5)