FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135727
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372280.9001561273265861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT40550.037.598461
TGGTATC41600.036.2301672
GTATCAA127550.024.9849951
GGTATCA72700.023.4318941
AGAGTAC94550.021.98772811
TATCAAC146900.021.5869182
CAGAGTA100850.020.88710410
ATCAACG153050.020.682053
TCAACGC152800.020.6591454
CAACGCA154850.020.4542035
TGCCGAC11450.020.4162355
AGTACGG102550.020.11131313
CGTCGGT1251.1245756E-619.80038553
AACGCAG160300.019.7918916
GAGTACG105700.019.59025412
GTACGGG112300.018.1933814
TACGGGG47050.018.00138515
CGCAGAG177150.017.9268138
ACGCAGA178200.017.8344577
TACGGGA51550.017.17679215
Produced by FastQC (version 0.11.5)