FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021724
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT75200.1497493689418216No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.14793724226978622No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63250.12595276044641243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC40000.045.6939242
GTGGTAT41300.044.2564961
GGTATCA77550.036.943391
GTATCAA139350.035.4147261
TATCAAC153550.032.1666832
ATCAACG155650.031.486923
TCAACGC156100.031.3955254
CAACGCA160000.030.5771265
AACGCAG164400.029.8880356
ACGCAGA183750.026.7363827
CGCAGAG184900.026.5920188
GCAGAGT203350.024.2211139
CAGAGTA125800.021.65435210-11
GAGTACG130900.020.50245312-13
GTCGAGG22400.020.110038
AGAGTAC113350.019.94609610-11
GTCGTAC4050.018.891921
GTACGGG141950.018.62212814-15
GTATTAG20000.018.2779331
GTACGAC4000.018.065043
Produced by FastQC (version 0.11.5)