FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021724
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342420.6818773791630125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT49200.038.650961
TGGTATC50300.037.24042
GTATCAA164700.024.0110131
GGTATCA90250.023.815031
AGAGTAC115250.021.7847511
TATCAAC183700.021.1576882
TGCCGAC13800.021.12543955
ATCAACG189300.020.4278343
TCAACGC189600.020.3491544
CAGAGTA125500.020.13718810
CAACGCA193500.019.9514395
AACGCAG195600.019.7776376
AGTACGG131150.019.12270213
GAGTACG135250.018.54301312
ACGCAGA214200.018.034767
CGCAGAG215700.017.8087888
GTACGGG145150.017.0313114
TACGGGG55350.016.9909715
GCAGAGT229700.016.796699
GCCGACG6200.015.96944255
Produced by FastQC (version 0.11.5)