FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6352757
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT109580.1724920377089821No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96120.1513043864262398No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69300.10908649583165231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT59000.045.6023251
TGGTATC62200.042.36622
GTATCAA208500.038.121581
GGTATCA114900.035.8458671
TATCAAC237350.033.2000472
ATCAACG242650.032.4398543
TCAACGC243400.032.3917734
CAACGCA245950.032.0386545
AACGCAG257250.030.713936
CGCAGAG278900.028.3120298
ACGCAGA281600.028.0561037
GCAGAGT310050.025.4667829
GTCGAGG31450.023.1073028
CAGAGTA192350.022.4809310-11
AGAGTAC179500.021.2728510-11
GAGTACG203750.020.97305312-13
AGTACGG196450.019.12394112-13
GTACGGG219050.019.12019314-15
AGTCGAG51300.017.3144847
GGCACCG12650.017.133259
Produced by FastQC (version 0.11.5)