FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5730056
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75940.1325292457874757No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65550.11439678774518086No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT61780.10781744541414605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC43550.042.844752
GTGGTAT47150.039.3979871
GGTATCA84300.037.2137221
GTATCAA157200.036.343021
TATCAAC176250.032.338582
ATCAACG176650.032.1215483
CAACGCA179000.031.6758175
TCAACGC179900.031.5646064
AACGCAG187000.030.3661616
ACGCAGA202950.027.9759987
CGCAGAG205950.027.5891198
GCAGAGT234150.024.3390059
GTCGTAC5850.023.2524361
CAGAGTA139850.022.71551510-11
GTCGAGG24700.020.99098
AGAGTAC129050.020.71412310-11
GAGTACG153900.020.37985412-13
GTACGGG162600.018.74069814-15
AGTACGG145200.018.29349712-13
AGTCGAG37150.017.960117
Produced by FastQC (version 0.11.5)