FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5730056
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377990.6596619649092434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC51350.036.7442052
GTGGTAT52550.036.7129971
GTATCAA183350.024.8122671
GGTATCA96050.024.2408331
AGAGTAC131650.023.27046611
TATCAAC197900.022.6819082
CAGAGTA140450.022.12591610
ATCAACG205850.021.5772723
TCAACGC209150.021.2060814
CAACGCA209950.021.1114435
AACGCAG217450.020.4324466
AGTACGG152550.020.0641113
GAGTACG158600.019.36826512
ACGCAGA237050.018.7433597
TACGGGG68700.018.73413715
CGCAGAG238800.018.6074628
CGCGGTA5300.018.16071543
TGCCGAC14700.017.95998855
GTACGGG169800.017.62090314
GCAGAGT258150.017.300349
Produced by FastQC (version 0.11.5)