FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6210054
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT112600.18131887419980566No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109240.17590829322901216No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64850.10442743332022555No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT64060.10315530267530684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT62800.047.9889761
TGGTATC65050.046.7189032
GTATCAA222350.040.2984541
TATCAAC251800.036.44442
GGTATCA115350.034.9706341
ATCAACG264650.034.562433
TCAACGC266300.034.3639644
CAACGCA269700.033.914995
AACGCAG280150.032.771296
ACGCAGA307550.029.8618687
CGCAGAG307250.029.8352178
GCAGAGT340900.027.0264669
CAGAGTA203950.023.6716810-11
GTCGAGG34750.022.6249628
AGAGTAC190000.021.7413810-11
GAGTACG224800.021.2398412-13
AGTACGG219550.018.6989912-13
AGTCGAG51350.018.6217297
GTACGGG253900.018.52109514-15
ATACGGG8650.018.1819521
Produced by FastQC (version 0.11.5)