FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481915
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68450.12486512468726713No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57620.10510925470387628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC43950.039.361072
GTATCAA137400.039.1980821
GTGGTAT45800.038.9815181
TATCAAC154200.034.9515342
ATCAACG153850.034.8376583
TCAACGC156600.034.3069954
CAACGCA157600.033.9544685
GGTATCA77150.033.059041
AACGCAG164200.032.5637866
ACGCAGA178750.029.8858157
CGCAGAG181200.029.4580088
GCAGAGT209400.025.7745489
CAGAGTA125550.022.22204410-11
AGAGTAC111000.021.95717210-11
GTCGAGG23300.021.3403668
GAGTACG133350.020.79483412-13
GTACGGG140450.019.50181814-15
AGTACGG126700.019.06873112-13
AGTCGAG30550.018.2236777
GTATTAG21350.018.1183411
Produced by FastQC (version 0.11.5)