FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481915
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346900.632808060686822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC47850.036.439952
GTGGTAT49300.036.395581
GTATCAA145450.023.7642381
GGTATCA82750.022.8806511
AGAGTAC104900.022.22958411
CAGAGTA113100.020.90965510
TATCAAC166300.020.6888082
ATCAACG169800.020.1949943
TCAACGC171350.019.9297084
TGCCGAC14850.019.63151755
CAACGCA174700.019.5768765
AGTACGG120350.019.30730213
AACGCAG181600.018.9532896
GAGTACG126700.018.33965112
ACGCAGA201050.017.065797
GTATTAG15600.016.9353681
GTCGTAC3750.016.8789161
CGCAGAG203900.016.8290988
GTACGGG139450.016.5050914
CGGGATA5000.016.49887517
Produced by FastQC (version 0.11.5)