FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5247117
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274480.5231063077114537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT56550.039.7680781
TGGTATC57700.038.135372
GTATCAA196650.025.1955451
GGTATCA102300.023.7322081
AGAGTAC141100.023.58224511
CAGAGTA147200.022.84807410
TATCAAC217800.022.6431182
ATCAACG225850.021.6370623
TCAACGC228950.021.3176334
CAACGCA230500.021.1852025
AACGCAG235700.020.7978886
AGTACGG161750.020.46938713
TGCCGAC14700.020.39263255
GAGTACG165750.019.99199912
CGCAGAG258500.018.8704688
ACGCAGA260300.018.8217937
GCAGAGT276250.017.6596589
ACGGGAT25800.017.58579816
GTACGGG186700.017.45407114
TACGGGG68300.017.39311815
Produced by FastQC (version 0.11.5)