FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5878634
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127740.21729537848418526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT56050.037.16111
TGGTATC56900.035.6230622
GTATCAA188600.024.2177961
GGTATCA101450.023.0534061
AGAGTAC127550.022.05656811
TATCAAC205450.021.967392
ATCAACG211900.021.14193
TCAACGC211400.021.1113324
CAGAGTA137850.020.96747410
CAACGCA215250.020.7060685
GTACGAC4900.020.2050673
AACGCAG221500.020.1461246
AGTACGG142950.019.50710913
GAGTACG147050.019.03818512
ACGCAGA246600.018.062277
CGCAGAG247850.017.9281758
GTACGGG161500.017.21542414
TACGGGG64650.017.14299615
GCAGAGT264000.016.8340119
CCGATCG1801.1557313E-716.80680346
Produced by FastQC (version 0.11.5)