FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6061699
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT118190.19497833858131194No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104540.17245989944403375No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74050.122160470191608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT55200.049.5107461
TGGTATC58600.046.781632
GTATCAA198000.038.167541
GGTATCA105000.038.0105251
TATCAAC224150.034.0360342
ATCAACG229200.033.100953
TCAACGC229500.033.0949944
CAACGCA232750.032.632875
AACGCAG245600.031.0639386
CGCAGAG265900.028.5616728
ACGCAGA269650.028.2750267
GCAGAGT296000.025.8141299
CAGAGTA172550.023.00365310-11
AGAGTAC154800.021.8253410-11
GAGTACG180350.021.74964712-13
GTACGGG196300.019.64701714-15
GTCGAGG34250.019.4812368
AGTACGG173850.019.1405212-13
AGCGTCA11400.018.2687173
TATACCG5000.017.8512945
Produced by FastQC (version 0.11.5)