FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6061699
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179920.29681447396183813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT65400.041.3168331
TGGTATC67950.038.894712
GTATCAA230950.025.2229651
GGTATCA122350.024.536581
AGAGTAC158150.023.12671711
TATCAAC260100.022.3945432
CAGAGTA169500.021.67545710
ATCAACG270900.021.2656123
TCAACGC271700.021.1595464
CAACGCA275750.020.9166665
AGTACGG176300.020.41809713
AACGCAG284150.020.3847336
GAGTACG182200.019.83254812
GTACGAC2550.019.410433
ACGCAGA314650.018.3920637
CGCAGAG314600.018.3546248
GTACGGG197650.017.93428214
TGCCGAC8750.017.91571655
TACGGGG79550.017.42295615
GCAGAGT334000.017.3150739
Produced by FastQC (version 0.11.5)