FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4669036
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.14236343433633838No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.1268784391467532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT38400.043.1704981
TGGTATC40450.041.295222
GTATCAA125400.037.252361
GGTATCA66800.035.1249961
TATCAAC140000.033.7600022
ATCAACG142100.033.1115343
TCAACGC141750.033.0733534
CAACGCA143700.032.5653955
AACGCAG148300.031.5839336
ACGCAGA165200.028.3507617
CGCAGAG168350.027.7947468
GCAGAGT192700.024.3915129
CAGAGTA110550.021.93130710-11
AGAGTAC98250.021.13000710-11
GAGTACG116100.020.66339512-13
TATACCG4150.020.4836645
GTCGAGG23000.020.1412878
AGCGTCA9900.019.749153
GTACGGG125300.018.8070414-15
AGTACGG111400.018.42603512-13
Produced by FastQC (version 0.11.5)