FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6560153
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165740.25264654650585133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC65400.039.5778052
GTGGTAT66600.039.545121
GTATCAA229700.024.836681
GGTATCA124200.023.4876441
AGAGTAC157350.023.4011811
CAGAGTA164350.022.58887510
TATCAAC254550.022.098362
ATCAACG262000.021.4040583
TCAACGC263100.021.2691884
CAACGCA267500.020.9580635
AACGCAG274100.020.492766
AGTACGG183200.019.93394513
GAGTACG190050.019.30242512
ACGCAGA301150.018.6345027
CGCAGAG302600.018.6017138
GCAGAGT323200.017.391629
GTACGGG213200.016.99973514
ACGGGTC9850.016.7497816
TACGGGG87500.016.62512415
CGCGGTA7900.016.36142543
Produced by FastQC (version 0.11.5)