FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314066
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT110200.2073741650931697No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100300.18874436260294847No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69850.13144360645878317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC54100.049.4189952
GTGGTAT56650.047.3476681
GTATCAA185100.037.914891
GGTATCA103800.034.3584631
TATCAAC211800.033.614712
TCAACGC218400.032.521344
ATCAACG219550.032.370053
CAACGCA221300.032.018345
AACGCAG234300.030.3688096
CGCAGAG251300.028.2078848
ACGCAGA259550.027.3936587
GCAGAGT271300.026.1905889
AGAGTAC141200.022.55848910-11
CAGAGTA158550.022.44872310-11
GTCGAGG28600.021.6947088
GAGTACG165900.021.18548212-13
CGTCAGA9100.021.0191785
GTACGAC6200.019.8813933
GCAGCGT10600.018.847821
AGTACGG167700.018.81666412-13
Produced by FastQC (version 0.11.5)