FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314133
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT103110.3111220943758141No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT101440.30608306908624366No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.25415395217995174No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66960.20204379244888482No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.12099695455794923No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC39340.11870374544413274No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT35060.10578935727685039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC47350.055.118872
GTGGTAT49250.052.4809531
GTATCAA180550.040.30981
GGTATCA96550.038.8347661
TATCAAC213400.034.777772
ATCAACG220400.033.7117843
TCAACGC220700.033.703464
CAACGCA224250.033.1130565
AACGCAG230500.032.288966
CGCAGAG247300.030.1257348
ACGCAGA253350.029.4403257
GCAGAGT263450.028.2781149
GTCGAGG24450.026.2468958
CAGAGTA156600.026.07944310-11
AGAGTAC151550.024.08817910-11
GAGTACG183300.022.18789912-13
TCGAGGT31350.020.3338899
GTACGGG199450.020.17851614-15
CGTCAGA6750.020.1504635
AGTACGG180900.020.05079812-13
Produced by FastQC (version 0.11.5)