FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500344
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT129010.36856377544607044No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT102770.2935997147708911No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.2525466068477841No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.18669593617084493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC49060.14015765307638337No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT41480.11850263859780638No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.10464685756599924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC44050.053.6466372
GTGGTAT44650.053.4077951
GTATCAA172550.039.6619571
GGTATCA93700.038.9687841
TATCAAC200350.034.4516682
TCAACGC206500.033.3844644
ATCAACG208700.032.991813
CAACGCA212150.032.495365
AACGCAG219700.031.552776
CGCAGAG238000.029.0527328
ACGCAGA243850.028.4428947
GCAGAGT253000.027.3630529
CAGAGTA150050.024.61239810-11
GTCGAGG27300.023.3509738
AGAGTAC142650.022.58227210-11
GAGTACG172550.021.32973712-13
GTACGGG182150.019.94906814-15
AGTACGG167800.019.08419612-13
AGTCGAG41150.018.8998187
TCGAGGT34050.017.7229189
Produced by FastQC (version 0.11.5)