FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4446769
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT123940.27871922287845396No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.15215541891202353No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58100.1306566632986782No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC49440.11118184911336748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT36900.044.2303921
TGGTATC37250.043.7002142
GTATCAA125100.038.1198771
GGTATCA70000.035.5200651
TATCAAC147000.032.729562
ATCAACG149100.032.325593
TCAACGC150050.032.0922434
CAACGCA152350.031.635655
AACGCAG156450.030.8880886
ACGCAGA172500.028.011957
CGCAGAG174100.027.7545178
GCAGAGT190700.025.3382669
CAGAGTA116500.022.61739710-11
AGAGTAC109750.021.5882410-11
GAGTACG128050.020.21247312-13
AGTACGG122950.019.08061212-13
GTCGAGG20250.018.8881538
GTACGGG138600.018.59748514-15
GTATTAG16450.017.569481
TATACCG3604.6929927E-1016.5284335
Produced by FastQC (version 0.11.5)