FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3855097
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT172250.4468110659731779No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC59790.1550933737854067No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.1531738371304276No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54390.14108594414096454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC31950.046.6945042
GTGGTAT31850.046.5796471
GTATCAA120650.041.0467531
GGTATCA60500.038.1526451
TATCAAC138050.036.269232
ATCAACG144950.034.3961033
TCAACGC147350.033.778184
CAACGCA148150.033.653165
AACGCAG154900.032.2415546
CGCAGAG167300.029.7964128
ACGCAGA169750.029.4168117
GCAGAGT182500.027.3842419
GTCGAGG15550.024.049958
CAGAGTA114600.023.01132410-11
AGAGTAC107200.021.80481110-11
GAGTACG127050.020.53894412-13
AGTACGG124600.018.70867312-13
GTACGGG139750.018.53582614-15
AGTCGAG24050.018.2007667
AGGGGGG53100.018.08738570-71
Produced by FastQC (version 0.11.5)