FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3855097
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360350.9347365319212461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC40600.038.616942
GTGGTAT41600.038.370771
GGTATCA76000.026.1450481
GTATCAA146200.026.015161
TATCAAC164050.023.0209562
AGAGTAC110600.022.70120411
CAGAGTA113750.022.14508210
ATCAACG174450.021.4734253
TCAACGC176650.021.2179874
CAACGCA177900.021.0810765
CGAATTA6850.020.87566615
AACGCAG183250.020.5392976
AGTACGG125600.019.90223313
GAGTACG127850.019.57348812
CCGAATT7700.018.92810214
CGCAGAG201650.018.6824158
ACGCAGA205400.018.285647
GTACGGG140100.017.76365714
GCAGAGT213650.017.6854889
TACGGGG56600.017.63671915
Produced by FastQC (version 0.11.5)