FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160922
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT192210.46194088713991754No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.20346452060384695No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79130.19017419696884488No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC55720.13391262801850165No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.13114881749766036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT40850.053.3821221
TGGTATC45900.047.2242852
GTATCAA174400.040.7286951
GGTATCA89550.039.5412141
TATCAAC199850.036.111162
CAACGCA208900.034.30265
TCAACGC211200.033.9692884
ATCAACG211350.033.9652863
AACGCAG212350.033.7252776
ACGCAGA234450.030.5055717
CGCAGAG240250.029.8225428
GCAGAGT256850.027.9947439
GTCGAGG23400.025.9699258
CAGAGTA162000.024.54292110-11
AGAGTAC152500.022.7414310-11
GAGTACG184100.021.3891912-13
TCGAGGT27700.020.5579959
AGTCGAG36950.020.1265287
GTACGGG196050.019.69545414-15
AGTACGG176600.019.49382212-13
Produced by FastQC (version 0.11.5)