FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160922
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405690.9750002523479171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT49550.040.7100521
TGGTATC53700.037.4420662
GTATCAA201200.026.3843021
GGTATCA103750.026.206571
AGAGTAC153800.024.15639911
TATCAAC223900.023.4268172
CAGAGTA159300.023.42595310
ATCAACG239600.021.8776453
TCAACGC240850.021.7705464
CAACGCA240600.021.7230325
AACGCAG242400.021.6405986
AGTACGG176900.020.90822613
GAGTACG183250.020.2439812
ACGCAGA268100.019.556357
CGCAGAG273750.019.1543358
GCAGAGT284850.018.4298959
GTACGGG197400.018.22146814
TACGGGG80850.017.6530815
TACGGGA86450.017.241215
ACGGGAT31050.016.91567216
Produced by FastQC (version 0.11.5)