FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23864
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.46094535702313105No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.34780422393563526No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT700.2933288635601743No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA670.28075762655045255No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.259805564867583No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGG550.23047267851156553No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.19694937981897417No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT470.19694937981897417No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC410.17180690579953067No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.15085484411666109No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTCTGCGTTGATACCTGTCTCT350.14666443178008715No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC330.13828360710693932No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC310.1299027824337915No Hit
GAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGG260.1089507207509219No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT260.1089507207509219No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT250.10476030841434797No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGACTGTCTCT240.10056989607777406No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT240.10056989607777406No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAG200.003184900963.753
GAGAAAC250.007703525551.02
TCCCTGA250.007703525551.02
TGGTATC409.1367133E-442.52
GGTATCA1400.039.4642831
GTATCAA2450.036.428571
TATCAAC2750.034.0000042
GCGAAGA451.3242534E-633.05555324-25
GTGGTAT550.004362094730.909091
GAAGGCG551.7994898E-730.9090920-21
ACGGGAA701.4242687E-930.35714116-17
ATCGTAT353.887399E-430.35714182-83
AACGCAG3150.029.6825396
TCAACGC3250.028.769234
ATCAACG3250.028.769233
CAACGCA3250.028.769235
ACGCAGA3350.027.9104487
GCAGAGT3400.027.59
CGCAGAG3400.027.58
CAGAGTA2400.027.44791610-11
Produced by FastQC (version 0.11.5)