FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59425
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.3315103071098023No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.2557846024400505No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.15986537652503155No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.1480858224652924No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT770.12957509465713082No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC670.11274716028607489No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1110643668489693No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10938157341186369No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.1026503996634413No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10096760622633572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTG200.003208448463.758
TATACTG200.003208448463.755
GTATCAA4500.051.0000041
TATCTTC250.00776011951.05
TATCAAC5200.047.4038432
TCAACGC5750.042.1304364
ATCAACG5800.041.7672423
CAACGCA5850.041.4102555
GTGGTAT1056.548362E-1140.476191
AACGCAG6300.038.4523856
TGGTATC1151.7462298E-1036.9565242
CGCAGAG7450.032.5167778
ACGCAGA7650.031.6666687
GCAGAGT7750.031.2580629
TTCTATC550.004414470430.909092
GTACAGT550.004414470430.909091
CTTCTAT550.004414470430.909091
GTCGAGG1251.6156264E-830.6000028
AGAGTCG3500.030.3571455
ATATCAA703.8988277E-430.3571411
Produced by FastQC (version 0.11.5)