FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165569
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.2216598517838484No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.1515984272418146No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.12623135973521613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG250.00777535551.015231
GGTACAA250.00777535551.015231
GACCGCT250.007784649350.999827
GACAGGT805.36289E-737.1986051
GTATCAA8050.036.439451
TATCAAC9350.035.9089622
GTGGTAT1900.035.800161
CGTCAGA601.5896783E-435.4165425
CAACGCA9900.033.484735
TGGTATC2050.033.1706162
TCAACGC10000.033.149884
GGTATCA4200.032.390621
ATCAACG10300.032.1843533
ATATCAA1605.456968E-1231.8845181
AACGCAG10400.031.8748876
CTTTACT550.00443720630.908982
GCGTCAG703.928899E-430.3570354
GTCAGAT755.8842596E-428.3332336
TCTCACT600.00678913728.3332332
CGCAGAG11750.028.2126668
Produced by FastQC (version 0.11.5)