FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45825
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.19858156028368795No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.15711947626841244No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.1265684669939989No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.11347517730496454No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT500.10911074740861974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATT250.007748826451.04
GTGGTAT857.2759576E-1250.01
ATATCAA354.7782765E-448.571431
GTATCAA3400.042.51
TGGTATC1003.8198777E-1142.52
TATCAAC4000.040.3752
ATCAACG4000.040.3753
AACGCAG4000.040.3756
TCAACGC4050.039.8765454
CAACGCA4150.038.915665
CGCAGAG4150.038.915668
ACGCAGA4400.036.7045447
GCAGAGT4600.035.1086929
CAGAGTA2400.029.21875210-11
GGGGGGC300.006773421528.33333646-47
GGTATCA1652.6193447E-1028.3333321
GAGTACG2800.025.04464312-13
AGAGTAC2300.024.02173810-11
ACGGGGA902.1307642E-823.6111116-17
GTACGGG3050.022.99180214-15
Produced by FastQC (version 0.11.5)