FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45825
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.5499181669394435No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.1331151118385161No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.10474631751227496No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACG150.0057492554.99781835
CAGACTC150.0057492554.99781842
GTGGTAT850.038.8644181
TGGTATC951.8189894E-1234.7734222
GGGGGAA400.007797282727.49890718
GGTATCA1502.1827873E-1123.8323883
GTATCAA2550.022.6709081
TATCAAC2700.021.4114132
TGACGCT650.0031938221.15300855
AGAGTAC1701.1459633E-1021.02857811
CAGAGTA1802.4738256E-1019.86032310
ATCAACG2950.018.6433283
TACGGGA750.007308163718.33260515
AGTACGG1957.203198E-1018.33260513
TCAACGC3050.018.0320724
GAGTACG2051.4042598E-917.43833412
CAACGCA3200.017.1868175
AACGCAG3300.016.6660066
GTACGGG2203.590685E-916.24935514
GTCGAGG1100.00368211714.9994058
Produced by FastQC (version 0.11.5)