FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165500
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.1770392749244713No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.12386706948640483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG250.007775893451.0143241
GAATAGG450.001656647537.788391
TAAGACT450.001656647537.788394
TCGACGT1202.8376235E-1035.415919
GTATCAA6600.034.7824941
GGTATCA3800.033.5620541
TATCAAC7450.033.0965422
GTATAAG652.5397787E-432.701491
GTCGACG1306.693881E-1032.691618
ATCAACG8150.030.2538953
CAACGCA8250.029.887185
TCAACGC8350.029.5292514
AACGCAG8350.028.5110026
GATGACG600.006789722528.3327277
TATAAGA808.566128E-426.569962
CGCAGAG9400.025.318618
ATTAAAC1201.2648748E-524.7986282
GCAGAGT9600.024.7911389
ATAAGAG1051.460428E-424.2925343
AGAGTCG4400.024.1545095
Produced by FastQC (version 0.11.5)