FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90632
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4630.5108570924176891No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC150.005767637454.99724216
CAAATGA150.005767637454.99724232
GACCATA303.4440964E-545.8816761
CGGTATA257.82003E-443.9977928
TTAAACG451.780536E-742.77563522
CTGCTTA504.0704072E-738.4980718
GCTTAAA504.0704072E-738.4980720
GAATATA300.001906383836.705341
GTGGTAT1650.036.7053381
ACCATAA300.00191156636.685072
CATAATG300.00191156636.685074
ACTGCTT604.0812665E-836.6648317
GCGGTCG300.001916759536.6648323
ATCACGT300.001916759536.6648352
CGGTCGG300.001916759536.6648324
TCGGTAT300.001916759536.6648327
GTCGGTA300.001916759536.6648326
TCACGTT300.001916759536.6648353
TGCTTAA558.5862666E-734.9982419
TGGTATC1850.034.2063522
Produced by FastQC (version 0.11.5)