FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104721
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4940.47172964352899616No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.22727055700384832No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.19957792610842143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAA600.050.40484219
GATACCA700.047.13179811
GTAACAG257.8327267E-443.9896834
CTTAAAC753.6379788E-1240.32387521
TTAAACG753.6379788E-1240.32387522
TGGTATC2250.036.675592
GTGGCCC300.00191985636.6580669
CAACTCA300.00191985636.6580665
TACCACT951.8189894E-1234.72869513
ACCACTG951.8189894E-1234.72869514
ATACCAC951.8189894E-1234.72869512
GTGGTAT2400.033.269061
GCTTAAA956.91216E-1131.83463920
GTATCAA9150.030.9934481
CGGGGGA801.4781108E-830.93024417
AGAGTAC7350.030.67307911
TACGGGT453.777427E-430.54838815
TACGGGC453.777427E-430.54838815
CTGCTTA1001.2732926E-1030.24290518
ACTGCTT1001.2732926E-1030.24290517
Produced by FastQC (version 0.11.5)