FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305
Sequences flagged as poor quality0
Sequence length91
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT48214.583963691376702No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG912.7534039334341904No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT832.5113464447806355RNA PCR Primer, Index 46 (95% over 21bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAGGCG631.9062027231467473No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT571.7246596066565807No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT541.6338880484114977No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGG421.2708018154311649No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT421.2708018154311649RNA PCR Primer, Index 46 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT330.9984871406959153No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGT320.9682299546142208No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG310.9379727685325264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC270.8169440242057489No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT250.7564296520423601No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGG220.6656580937972768No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGGCGG220.6656580937972768No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGG180.5446293494704992No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA170.5143721633888049No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTAAGGCGAAT160.4841149773071104No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTGT160.4841149773071104Illumina PCR Primer Index 8 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCGT160.4841149773071104No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGG160.4841149773071104No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTATGCCGT150.45385779122541603No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGG140.4236006051437216No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTGTT140.4236006051437216No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGG130.39334341906202724No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGG120.3630862329803328No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGG100.30257186081694404No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAG100.30257186081694404No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCTT100.30257186081694404No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCGT100.30257186081694404No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGTCTT100.30257186081694404No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTT100.30257186081694404No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTATGCCGG90.2723146747352496No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGG90.2723146747352496No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGG90.2723146747352496No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGT90.2723146747352496No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT90.2723146747352496No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC90.2723146747352496No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGGCGT90.2723146747352496No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGG90.2723146747352496No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTA80.2420574886535552No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGG80.2420574886535552No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGTCTT80.2420574886535552No Hit
TGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAGGC80.2420574886535552No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT80.2420574886535552No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGC70.2118003025718608No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGT70.2118003025718608No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT70.2118003025718608No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCCT70.2118003025718608No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT70.2118003025718608No Hit
GTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCGAATCT70.2118003025718608No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGT70.2118003025718608No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGG60.1815431164901664No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGG60.1815431164901664No Hit
GTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT60.1815431164901664No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGATGGCGG60.1815431164901664No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCC60.1815431164901664No Hit
GCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG60.1815431164901664No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATTGCGT60.1815431164901664No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTATGGCGG60.1815431164901664No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCG50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTGCGG50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTTTTCCGT50.15128593040847202No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTGTTCT50.15128593040847202Illumina PCR Primer Index 8 (95% over 21bp)
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG50.15128593040847202No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCGTGT50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTTTTGCGT50.15128593040847202No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGGCTT50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGCATCTCGTATGCCGT50.15128593040847202No Hit
GTGTATAAGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTAAGG50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGGCGT50.15128593040847202No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCG50.15128593040847202No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGC50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATTCCGT50.15128593040847202No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTTGT50.15128593040847202No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGT50.15128593040847202No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCG40.1210287443267776No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGTCTTGT40.1210287443267776No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGGTTGGCGG40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTGGTTTGCCGT40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTGCCT40.1210287443267776No Hit
GTGTATAAGAGACAGTCTTATACACATCTCCGAGCCCACGAGACTAAGGC40.1210287443267776No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG40.1210287443267776TruSeq Adapter, Index 4 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTTGCGG40.1210287443267776No Hit
CAGATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT40.1210287443267776No Hit
GAGATGTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTAAGG40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCCG40.1210287443267776No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCG40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATGTCGTATGGCGT40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCCT40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATTCCGT40.1210287443267776No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGTCGT40.1210287443267776No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTTCT40.1210287443267776Illumina PCR Primer Index 8 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGT40.1210287443267776No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGTTTGGCGG40.1210287443267776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2550.050.0000041
TATACAC2600.049.0384643
TTATACA2600.049.0384642
TACACAT2900.045.4310345
ATACACA2900.045.4310344
CGTTTGC208.66096E-442.540-41
ATAAGAG408.0311095E-442.55
GTGTATA408.0311095E-442.51
TATAAGA408.0311095E-442.54
AAGAGAC408.0311095E-442.57
TAAGAGA408.0311095E-442.56
GTATAAG408.0311095E-442.53
CTGCGTG208.66096E-442.554-55
TCTGCGT208.66096E-442.552-53
TCTTCTG208.66096E-442.550-51
TGTATAA408.0311095E-442.52
CTTCTGC208.66096E-442.550-51
CACATCT3250.040.538467
CATCTCC3250.040.538469
ACATCTC3250.040.538468
Produced by FastQC (version 0.11.5)