FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448099
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62390.4308407090951654No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41130.28402754231582233No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36920.25495494437880284No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.21973635780426615No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.19867426191165108No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT24700.17056844870412866No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.1678752626719582No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22120.1527519872605395No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17680.12209110012506051No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17610.12160770776031198No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15120.10441275078568522No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.10199578896194252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG150.00102998384.992339
AGCGTCA700.066.793533
TCGAAAT401.9556319E-763.7574652
GCGTCAG850.055.006444
AAGTACG6400.051.8118861
CGGTATC354.824625E-448.5771142
AGTATCA6150.047.0054861
CTGTGCG1900.046.9694449
TGGTATC38450.046.429362
GTGGTAT35200.045.4108851
CGTCAGA1051.8189894E-1244.529025
CCCTATG505.4433658E-542.5123141
CGAAATC603.206851E-642.5049743
GCCCAGA903.189234E-837.78222
GGTATCA54850.036.738081
ATATCAA22000.034.5895651
TAGACTT1008.059942E-834.003985
GTATCAA101000.033.083841
CCTAGAC1051.237222E-732.3847433
GTCGAGG23700.031.7376448
Produced by FastQC (version 0.11.5)