FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460526
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.13844327317692393No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.12132615235880771No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT15660.10722164480467995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12100.036.8878171
GTATCAA41550.036.626131
TATCAAC47500.033.3783232
TGGTATC14450.033.2399142
GGTATCA22500.032.6845551
CAACGCA49250.031.9311855
ATCAACG50100.031.3915883
TCAACGC50600.031.1632654
AACGCAG51000.030.8355066
CGCAGAG56300.027.6269388
CGTCAGA2950.027.3748135
TACGGGT2500.027.2019184
ACGCAGA57800.026.9099777
GCAGAGT60400.026.033049
GTACTAG3250.024.8513091
ATACGGG1601.828339E-723.9112111
GTCTATA2053.4633558E-922.8096081
AGCGTCA3550.022.7496413
ACGGGTA1502.789271E-622.6682645
AGTCGAG10500.022.665167
Produced by FastQC (version 0.11.5)