FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403845
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.14852067001698907No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.14025764952683523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13000.041.526541
GTATCAA45100.035.06161
TGGTATC16050.033.369172
TATCAAC51450.032.1376532
GGTATCA25150.032.1130071
CAACGCA52800.030.831835
ATCAACG53400.030.6456893
TCAACGC55200.029.5683164
AACGCAG56650.029.0365816
ACGCAGA61950.026.3437717
CGCAGAG61750.026.220758
GCGTCAG3650.025.6191234
AGCGTCA3650.024.4554883
GCAGAGT67500.023.987139
GCCTACG1652.4613655E-723.18591
CTAGTAC3700.022.9761983
GTCGTGC1852.926754E-822.9712898
CAGAGTA38050.021.3880510-11
CTGTGCG4000.021.2484449
AGAGTAC34900.021.24844210-11
Produced by FastQC (version 0.11.5)