FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403845
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37960.270400222246758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGT300.001927857836.6585666
GTGGTAT13750.036.228141
TGGTATC15800.031.5085532
TGCGAAT400.00788220827.4939257
GTATCAA48150.025.3208121
AGAGTAC29550.025.1258311
CAATGCG1101.19289325E-824.9971519
CAGAGTA31050.024.08914810
TGCGACG1151.9124855E-823.91116722
CGACGAG1203.004061E-822.9148724
TATCAAC54150.022.2983672
CAACGCA56000.021.3085525
ATCAACG55900.021.2543563
GGTATCA25200.021.1870941
CCGTCGA650.003240906221.1529439
AACGCAG57850.021.1491766
TCAACGC56600.021.084174
ATGCGAC1701.2551027E-1021.02851321
AGTACGG34900.020.95898813
GTACTAG2650.020.7708511
Produced by FastQC (version 0.11.5)