FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898191
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT23220.1223269944910707No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.12037777020331462No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.11284428173982493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16700.037.668382
GTGGTAT17000.036.7545931
GTATCAA46150.034.722651
TATCAAC54350.031.1254252
ATCAACG54850.030.76423
CAACGCA54850.030.5317255
GGTATCA26200.030.1755241
TCAACGC56050.029.8780564
AACGCAG59000.028.4561946
AAGTACG2700.026.7626041
CGCAGAG64900.025.6032528
ACGCAGA66000.025.112147
TATATCG900.001710492423.613445
GTCGAGG8900.022.9199878
GCAGAGT73700.022.8344619
GTACTAG4700.022.6092071
TCGAGGT9050.022.5400949
GTACCGT1050.00418525320.240096
TACGACC1050.00418525320.240094
TATCGGC1507.1436334E-519.8352872
Produced by FastQC (version 0.11.5)