FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898191
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87290.4598588866979139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG401.9026057E-434.37317328
GTGGTAT17300.034.2096371
TGGTATC17200.033.7443432
TCGCGTA901.448565E-624.44314423
GTATCAA53650.023.7556761
CGGGTAA600.00203664522.91544717
CCGGTAA752.8725987E-422.00114838
GCGCGTA752.8741348E-421.9994113
GGTATCA29700.021.5951181
TACGACC650.003241022621.1532784
AGAGTAC36100.020.94819311
ATGCGAC1207.6015385E-720.62390321
TATCAAC61700.020.5078262
GTCTAGC2053.6379788E-1220.1415791
CAACGCA61750.019.859725
AACGCAG62550.019.8244726
TCGTCTA1251.123848E-619.80051454
ATCAACG62500.019.7145883
CAGAGTA38850.019.60693710
TCAACGC63150.019.4650364
Produced by FastQC (version 0.11.5)