FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141692
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70880.33095328366543836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18000.035.9241871
TGGTATC19400.031.6148932
ACGCGTT453.7963653E-430.55591811
CGTAACG507.0353487E-427.4990423
GTATCAA55900.023.6276761
GCGACCG600.002036512822.91586718
GACCGTA752.8740027E-421.99974620
GGTATCA32300.021.7234731
GACGCGT650.003240428421.15409910
TATCAAC64050.020.3539072
ATCAACG65700.020.0083163
TCAACGC66700.019.7458824
CAACGCA67300.019.4849435
TCGGTAT856.6848984E-419.41244942
AGAGTAC40000.018.90647511
AACGCAG70050.018.7980616
CGATCGG750.007411415718.33440839
CAGAGTA42800.018.05512210
GTCTATA2600.017.991491
ACACGCG1101.8657891E-417.50102637
Produced by FastQC (version 0.11.5)