FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919579
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48440.25234699900342733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16200.036.34991
TGGTATC16200.035.3160062
GTATCAA59100.025.7479151
GGTATCA30800.024.1222321
TATCAAC64850.023.0310172
AGAGTAC41750.022.46049311
TATACCG1757.2759576E-1221.9936945
CAACGCA69000.021.5155725
ATCAACG69500.021.3306733
TCAACGC69600.021.2571854
AATACGC650.003245922521.1477835
CAGAGTA46200.021.01135610
AACGCAG71950.020.6716276
TGTGCGA956.0635255E-520.26262910
AGTACGG47500.019.62580313
CGCAGAG81150.018.3309448
GATACCG750.007425916418.328085
ACGCAGA81000.018.2611487
TACGGGG28900.017.9844315
GAGTACG52000.017.92741812
Produced by FastQC (version 0.11.5)