FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459038
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.251329985922231No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.19224996196123748No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.12857787117264938No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.12069596542379293No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16910.11589828366361946No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16520.11322528954009423No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA14630.10027154878762583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG200.003219831763.7691041
TGGTATC19200.055.1280332
GTGGTAT20050.050.039931
GGTATCA38050.038.65811
GATCGAC450.00166326637.7774437
GTATCAA70750.036.2334671
TATCAAC86650.034.2912252
AAGTACG6300.033.065461
CTACGAC901.3583558E-633.062063
TCAACGC94650.031.4837654
ATCAACG94600.031.4543913
TATCGGC952.0717325E-631.3219532
CAACGCA94950.031.2947525
AACGCAG101900.029.3689066
GTCGAGG15200.028.7990868
GTCGTAC600.006796225428.3418251
TACGACA905.1479503E-528.339884
AGTCGAG19800.026.401287
CGCAGAG114150.025.9875058
GTCTAGA2500.025.507641
Produced by FastQC (version 0.11.5)