FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459038
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61410.42089376698893377No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19940.13666539185408466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23300.042.8434262
GTGGTAT23600.042.5522841
GGACGCG350.004099266631.42970842
CTACGAC350.004099954431.428633
TTGGCGC400.00786979427.5028813
GTCGAAC507.035577E-427.49816526
CTATACG705.711272E-627.4981654
AGACGCG858.801271E-725.88062927
AGCGTTG550.0012271924.99833330
GTATCAA81300.024.9074521
CGAATTA2550.024.80566815
GGTATCA43100.023.8631533
AGAGTAC55550.023.51731511
AGTGCGC600.002034551722.91906510
ATCGCTC600.002036580822.91513827
CAGAGTA58150.022.56040210
CCTATAC1605.638867E-1122.3437923
GTCTATA2100.022.2726381
TATCAAC91850.022.065912
GCGTAGA1003.6013025E-622.0106071
Produced by FastQC (version 0.11.5)