FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163051
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.2440954057319489No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.2306026948623437No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.2306026948623437No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.15577948003998748No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1447399893284923No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.125114228063612No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.1128481272730618No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.11223482223353429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA357.719427E-872.8608253
GTCTGGC250.007782771751.002581
AGTATCA509.089308E-751.002581
TGGTATC2650.048.115642
GTGGTAT2500.047.6024061
GCGTCAG551.753182E-646.365984
GCCGGCG255.441728E-542.50214846-47
GCATGCA409.293537E-442.5021441
TATCAAC12550.040.6395032
GTATCAA9550.040.4994281
ATATCAA3250.039.2327541
CTACTCC450.001658450837.7796864
AAGTACG709.1964175E-636.4304121
CAACGCA14350.035.5418665
TCAACGC14600.034.9332734
ATCAACG14700.034.40653
CCGGCGT250.002785381434.00171746-47
AACGCAG15500.033.7275126
GTCATTC550.004435833530.9106521
TCGAGGT2500.030.5921639
Produced by FastQC (version 0.11.5)