FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163051
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18851.156079999509356No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.2588147266806091No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.15516617500045998No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.11775456758928188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT150.00576250755.02947213
GTTTAAG351.5677269E-647.1247181
GTGGTAT2600.043.3486981
TGGTATC2950.038.205632
TGTGACA300.001915734836.68631410
GTCACAT350.004090973631.42611719
CTGGAGT453.7831304E-430.5531721
GTATCAA13300.028.9362261
AGTCACA400.00785664627.49785218
CCGTCAG606.288419E-527.49785240
CTAAGTC400.00786842227.4894184
TATCAAC14750.026.6507572
AATGTAC858.7453554E-725.8723953
CAGAGTA10450.025.80329510
TGTATAA651.08619744E-425.3748472
GTGTATA651.08619744E-425.3748471
AGAGTAC10750.025.33915311
GACATTA550.001220411225.0057230
CGGGTCC550.00122259824.99804536
TTATCCC550.001224788124.990384
Produced by FastQC (version 0.11.5)