FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448293
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.14164843082537537No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.13116421626034758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.037.993371
GGTATCA7450.036.510981
TATCAAC16050.035.483752
AGCGTCA1008.045208E-834.0008473
ATCAACG17250.032.7689323
CGATACC652.550509E-432.6931234
CAACGCA17300.032.4285555
TCAACGC17550.032.2087824
GTGGTAT3850.032.0137861
AAGTACG1003.0882584E-629.7507421
AACGCAG18850.029.5365456
TGGTATC4600.028.642022
CGTTGAA300.00680396928.33403818-19
CGCAGAG19800.028.1162538
GTACTAG957.43869E-526.8427731
ACGCAGA20950.026.5728787
GCAGAGT21600.025.7732339
CAGAGTA12800.024.0701810-11
TACTACC900.001709195223.6116982
GTCTTAC1703.2768912E-722.500561
Produced by FastQC (version 0.11.5)