FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448293
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16160.3604785263209553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCACG300.001915664336.7005461
CATTGCG558.682109E-735.00106429
GTCGTCA658.371899E-833.84718324
GTGGTAT4750.033.609971
GCTCGTC701.6047488E-731.4295339
TGGTATC5000.030.250922
TGTGCGC653.1991895E-629.61628710
ATTGCGC653.1991895E-629.61628730
GTATTAT400.007832712527.5254081
TCGCACT606.307993E-527.50083732
CGGTATA606.307993E-527.50083728
TATACTG507.0239307E-427.5008375
TTCGCAC400.00786692627.50083731
GTCGGTA606.307993E-527.50083726
GCGCCAA759.732816E-625.66744813
CTGTGCG759.732816E-625.6674489
TCGTCTA759.741247E-625.66458554
GTATCAA16950.024.6835541
CTAGTAC1451.4551915E-1124.6559243
TCAGCGC901.444796E-624.44246344
Produced by FastQC (version 0.11.5)