FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287590
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.2628742306756146No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.2531381480579993No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.1439549358461699No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.11161723286623318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5250.047.761222
TACACTC452.9214316E-547.2215425
GTGGTAT5000.045.899341
ACCGATC559.554549E-538.6290938
GTACCGC450.0016610937.7772376
GTATCAA18050.035.3180541
GGTATCA10400.032.691841
TACTAGG652.5488329E-432.691842
TATCAAC20750.032.360982
ATCAGCG550.004446611730.9032757
ATGTCGT353.9411444E-430.35670940-41
TTGGTAC703.9362902E-430.3567093
TAATCAA703.9403047E-430.3514279
CTGTGCG703.9403047E-430.3514279
CTCTATT853.4821816E-529.9995692
CAACGCA22300.029.9210975
ATCAACG22950.029.0736583
TCAACGC23100.028.8848694
AAGTACG1203.972582E-728.3329281
CCTAGTA755.895284E-428.3329282
Produced by FastQC (version 0.11.5)